Context
Deregulated kinase activity is a frequent cause of disease, particularly cancer, where kinases regulate many aspects that control cell growth, movement and death.
Transcription factors have a role in human health such as in energy metabolism, cellular proliferation, and cellular differentiation. They are involved in human diseases such as in male infertility, prostate cancer, and leukemia.
Strategy
The production strategy involves two phases, a high-throughput expression screening step followed by the optimization of the conditions of production and purification.
Both Escherichia coli and baculovirus/insect cells expression systems were used to produce recombinant proteins including kinases and transcription factors, for various medical applications.
Status
List of protein kinases that have been produced: AURORA_2, RPS6KB2, PRKAA2, SYK, IKBKB, PTK2_var1, TEK, JAK3, FLT_1, KDR, ROCK_2
List of transcription factors that have been produced: NFκB p60, NFκB p55, BRCA1, BRCA2, E2F, c-myc, Mad, Max, ETS-domain, RARα, MyoD, Myogenin, E12, MRF4, Id (1 to 4), SRF, MEF2, Pax3, BMP2, GHF-1/Pit1, PPARα, PPARβ, PPARγ, Sp1, AML1/CBFβ, Egr-1, c-myb, C/EBPα, C/EBPvarα, Hox, MZF-1, CREB/ATF, PU.1, P300/CBP, Smad (1 to 4), P63, P73, STAT, Kruppel-like factor, Swi/SNF, Jun, Fos, AP-1, GATA(several members), NF-Y, DP-1, KU 70/80
All the transcription factors produced are involved in human diseases.